RAPD Marker Conversion into a SCAR Marker for Rapid Identification of Johnsongrass [Sorghum halepense (L.) Pers.]
نویسندگان
چکیده
منابع مشابه
Population genetics structure of glyphosate-resistant Johnsongrass (Sorghum halepense L. Pers) does not support a single origin of the resistance
Single sequence repeats (SSR) developed for Sorghum bicolor were used to characterize the genetic distance of 46 different Sorghum halepense (Johnsongrass) accessions from Argentina some of which have evolved toward glyphosate resistance. Since Johnsongrass is an allotetraploid and only one subgenome is homologous to cultivated sorghum, some SSR loci amplified up to two alleles while others (pr...
متن کاملGlyphosate resistance in perennial Sorghum halepense (Johnsongrass), endowed by reduced glyphosate translocation and leaf uptake.
BACKGROUND In a large cropping area of northern Argentina, Sorghum halepense (Johnsongrass) has evolved towards glyphosate resistance. This study aimed to determine the molecular and biochemical basis conferring glyphosate resistance in this species. Experiments were conducted to assess target EPSPS gene sequences and (14)C-glyphosate leaf absorption and translocation to meristematic tissues. ...
متن کاملControl of Johnsongrass (Sorghum halepense) in tall fescue (Festuca arundinacea) stands
Johnsongrass is a nonnative invasive species introduced as a forage crop from the Mediterranean region (Miller and Miller 1999). This perennial species has become naturalized and is found in 47 of the 50 United States (Alaska, Minnesota, and Maine being the exceptions) (USDA 2004). Johnsongrass has become problematic along roadsides in Kentucky due to its aggressive and prolific nature and rapi...
متن کاملDevelopment of a transposon-based marker system for mutation breeding in sorghum (Sorghum bicolor L.).
Under certain circumstances, transposable elements (TE) can create or reverse mutations and alter the genome size of a cell. Sorghum (Sorghum bicolor L.) is promising for plant transposon tagging due to its small genome size and its low content of repetitive DNA. We developed a marker system based on targeted region amplification polymorphisms (TE-TRAP) that uses the terminal inverted repeats (...
متن کاملComparison of RAPD Marker Patterns
An analysis of morphological, physiological, and molecular data from five cactus fruit varieties from Mexico and Chile, two ornamental Texas accessions, and one vegetable accession from Mexico are presented. Molecular analysis was performed using the RAPD (random amplification of polymorphic DNA) method. Phenotypic and molecular analyses distinguished ornamental, vegetative, and fruit market ac...
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ژورنال
عنوان ژورنال: Notulae Botanicae Horti Agrobotanici Cluj-Napoca
سال: 2013
ISSN: 1842-4309,0255-965X
DOI: 10.15835/nbha4119001